Profile Photo
Lisa Borghesi, Ph.D.

contact

Office: E1058 BST

Lab: E1011 BST

Phone: 412.383.7074

Fax: 412.383.8098

Email: borghesi@pitt.edu

Website: Borghesi Lab

education

Ph.D. - University of Connecticut

academic affiliation

Associate Professor, Department of Immunology

Director, Immunology Flow Cytometry Facility

Assistant Director, Immunology Graduate Program

research interest

Mechanisms of E47-mediated leukemogenesis

Transcriptional regulation of B and T lymphocyte production

Hematopoietic stem cell integrity and lineage fate decisions

lab members

Postdoc
Yujuan Wang, PhD

Graduate Student
Patti Santos

Undergraduate
Josh Bowman

grant support

Title: The role of E47 in uncommitted hematopoietic progenitors
Agency: NIH
Role: PI
Funding Period: 2009-2014

About Research

Dr. Borghesi's research focuses on the molecular mechanisms underlying B and T lymphocyte development with specific emphasis on the transcription factor E47. E47 is an essential regulator of lymphocyte formation and function, and dysregulation of E47 due to gene deletion or translocation is associated with fatal immune deficiencies and cancer.

Immature lymphomas represent an appreciable proportion of human hematopoietic malignancies yet the precise mechanisms by which E47 disruption causes lymphoid cancer remains unknown. The lab uses a two-pronged approach to identify downstream targets of E47 activity in both normal lymphocytes and leukemic clones. Knowledge about the molecular mechanisms underlying E47-mediated oncogenesis provides raw insight into basic cell biology and offers a rational foundation for targeted disease therapy.

The lab's most recent findings demonstrate a requirement for E47 in the hematopoietic stem cell compartment. That E47 is linked to cancers of multiple lymphoid compartments raises the possibility that disruption of E47 in stem cells acts as a first lesion that renders cells susceptible to secondary, transforming events in a lineage-dependent manner. Dr. Borghesi's publications appear in the Journal of Experimental Medicine, PNAS, and Nature Immunology, and are frequently ranked by the Faculty of 1000.

Selected Publications

Yang Q, B Esplin & L Borghesi E47 regulates hematopoietic stem cell proliferation and energetics but not myeloid lineage restriction. Blood (2011) 117:3529-3538

Martincic K, S Alkan, A Cheatle, L Borghesi & C Milcarek Transcription elongation factor ELL2 directs Ig secretion in plasma cells by stimulating altered RNA processing. Nat Immunol (2009) 10:1102-1109

Yang Q, L Kardava, A St Leger, K Martincic, B Varnum-Finney, I Bernstein, C Milcarek & L Borghesi E47 controls the developmental integrity and cell cycle quiescence of multipotential hematopoietic progenitors. J Immunol (2008) 181:5885-5894

Borghesi L, J Aites, S Nelson, P Lefterov, P James & R Gerstein E47 is required for V(D)J recombinase activity in common lymphoid progenitors. J Exp Med (2005) 12:1669-1677

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