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Tip #100. Staining media.
We routinely use the following staining media, simple to prepare:
500 ml Dulbecco's PBS, Mg/Ca-free, pH 7.2
3% newborn calf serum
0.02% sodium azide
1 mM EDTA, pH 7.5
Works for both mouse and human cells.
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Matt Barry
Senior Administrator
Department of Immunology
On managing the finances of a department core... |
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1. Your role in managing the FACS facility finances means that you take the heat for the fee structure. How are the fees established?
The fees are in line with what has been established at the University of Pittsburgh Cancer Institute (UPCI). When they vacated the BSTWR for the new building in Shadyside, Oakland was essentially left without a Flow Facility, so ours replaced that. Furthermore, we were able to forecast what would be needed to maintain the equipment and the facility, pay for a Flow Facility manager, etc., and luckily the established fee structure supported that.
2. Does the Department make a profit?
No. The facility has been mostly a self-supporting entity, although recent Flow Facility equipment upgrades required us to dig into our development fund as opposed to charging all of the costs to the facility. We also anticipats a high maintenance bill for the FACSAria, as well as the usual maintenance bill for the LSRII.
3. Unexpectedly, about half of the complaints we hear about the facility come through you. Are you thinking about changing your open door policy?
The only regular complaint I hear is that certain individual flow users BSTWR badges stop working in the card access slot for no apparent reason. I have been talking with the security personnel as to why this is happening.
4. Does the candy jar on your desk appease ruffled FLOWers?
The candy jar has been empty the last several days. It may be coincidence, but I haven't had a lot of people visiting me over this same period of days
5. What's your favorite cat clip on YouTube?
I only know of two, both of which had been emailed to me from a couple of friends who also have a cat [here is one http://www.youtube.com/watch?v=2GWPOPSXGYI] . Plus, I'm sure Lucy would be upset if she knew that I found anyone else's cat the least bit entertaining.
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