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  • 12/10/2013

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  • 6/25/2013

  • Free software for flow cytometry analysis.
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  • 3/20/2013

  • Mulitcolor Flow Cytometry Seminar March 26 1:00pm-3:00pm BST 10th floor conference room.
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  • 2/4/2013

  • Unexpected autofluorescence from certain bullet tubes.
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  • 1/8/2013

  • Mass Cytometer-CyTOF - special seminar sponsored by Starzl Inst.
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    News Archive...
           LSR II Reservations    |    Fortessa    |    Protocols     |    Sample Prep    |    Instrument Fees

    Tip #751. Autofluoresence and compensation.
    Autofluorescence is intrinsic cellular fluoresence that can contribute to background signals, and interfere with sensitive detection of low levels of antibody staining. For optimal resolution of stained populations, try to choose compensation controls that have the same level of autofluorescence. Compensation beads will not be the best choice because of their low intrinsic fluorescent properties.

    Olivera J Finn
    Department of Immunology

    On creating life-changing opportunities through persistence and academic excellence...

    Academic positions are competitive enough for students born in Western countries. As a young woman in [the former Republic of] Yugoslavia, how did you achieve first a degree at Stanford and then a position at Duke?

    Actually, I first began my undergraduate training in this country. I studied at the Inter-American University of Puerto Rico while my husband, Seth, was serving there in the Coast Guard. I think that gave me a dual handicap Ė not only was I a foreign student from East Europe, but then I went to a second rate university. I made up for that by maintaining a 4.0 grade point average the entire time and finished the university in record time of 3 years. Even at a second rate university that is not so easy to do, especially not in a foreign language. I studied there in a mixture of English and Spanish, none of which I spoke well when I started. But at the end I did and I could put on my graduate school application that I spoke three languages fluently, Serbo-Croatian, English and Spanish.

    Because I graduated before my husband was finished with his Coast Guard duty, I applied and got a job in the Department of Radiology at the University of Puerto Rico School of Medicine. A wonderful professor, Dr. Chiriboga, needed a lab assistant for a course he was teaching that spring. My job was to grow cell lines for the students, plate them in tissue culture dishes and help the students do radiation survival studies with these cells. That was not very much work, so in my spare time I worked with a young geneticist in the lab next door to mine. He had just gotten back from a postdoc at Stanford. He was analyzing amniocentesis samples for chromosomal aberrations and I helped him prepare chromosome spreads. Two years later, he was one of my references when I applied to Stanford.

    My husband was discharged from the Coast Guard in June of 1972 and in September we started life at Stanford. He returned to the Masterís degree program in the Department of Communications that he had interrupted when he was drafted, and I found a job as a technician in a radiobiology lab (on Dr. Chiriobogaís reference - see the connection with my small teaching assistant job!). I have learned that everything you do in life provides you with special experience that sooner or later becomes useful.

    What I didnít know was that Dr. Henry Kaplan, my supervisor, was a very famous radiologist who had just come up with a cure for Hodgkins Disease. As I was starting in his lab, NCI was putting up a whole new building on Stanford campus, just for him. I worked hard and independently and when a year later I told him that I would like to enter the Stanford Microbiology Graduate Program and study immunology, he gave me his full support. I was a little worried when he did not answer immediately, but it turned out that he was calculating how old exactly my baby was going to be when I started, since I was standing in front of him in the advanced stages of my first pregnancy.

    My son was 7 months old when I started and my daughter was born three months before my defense. At that time, my husband had a job producing local TV news in San Francisco. He had gotten the job just before I started graduate school. Once I finished, it was his turn to go to school again. I started a postdoctoral fellowship in the Department of Oncology at Stanford and he went back to the Communications Department where he had gotten his Masters, to finish his PhD.

    When Seth was done three years later, we looked for a place that could accommodate two academic positions and provide a good environment for our children (8 and 3 at that time) to grow up. We found that in North Carolina and in 1982 Seth took an Assistant Professor tenure track position at UNC and I took a non-tenure track position at Duke. This was a compromise on my part, but all it did was cost me a little bit of time. Two years later I was moved onto tenure track.

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