The cytometer is working. Pitt Flow Cytometry FACS Facility - Department of Immunology.
You are not signed in.

[ Sign In ]

Instructions for New Users

Search this site:
(Powered by Google)
ANNOUNCEMENTS &
HIGHLIGHTS


  • 11/13/2009

  • How many events is enough? Are you positive?
    Read more




  • 10/13/2009

  • Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
    Read more




  • 8/13/2009

  • Multiplexed labeling of samples with cell tracking dyes facilitates rapid and accurate internally controlled calcium flux measurement by flow cytometry.
    Read more



  • 8/4/2009

  • Cell Type Specific Applicability of 5-Ethynyl-2'-deoxyuridine (EdU) for Dynamic Proliferation Assessment in Flow Cytometry.
    Read more




  • 6/25/2009

  • New to Flow?
    Check this out-- Flow Cytometry: A Basic Introduction.

    Read more




    News Archive...
              LSR II Reservations       |       Protocols       |       Sample Prep       |       Instruments & Fees

    Tip #99. Bad habit: Exploiting the "Duplicate without data" function on DIVA to re-use compensation controls from another experiment.
    Accurate compensation relies on the brightness of controls and on the antibody lot. Different lots of antibodies have different degrees of fluorescence (due to distinct fluorochrome:protein ratios). Duplicate old experiments and sure, you'll get the same proportion of CD3, CD4, CD8 and CD19 cells, the big populations that are easy to resolve. What you won't get is accurate, reproducible resolution of rare populations - Tregs, tetramer positive cells, hematopoietic progenitors…

    Dewayne Falkner
    Manager, Flow Cytometry Facility
    Department of Immunology

    On the transition from bench to Facility Director...

    1. How did you become facility manager?

    When I heard that the Immunology Department was purchasing a flow cytometer I asked Penny Morel if I could be the one to go to training. I worked part time in the facility until there was enough work to keep me busy full time. With the help of Penny [whose grant writing efforts funded the Aria] and Olja [Department Chair] I transitioned from the lab to facility manager.

    2. What is your vision for the flow cytometry facility?

    I would like the facility to be a place where results are easily acquired and a resource for flow cytometric information.

    3. What are the main challenges with running a core facility?

    The facility has been growing since the LSR II was installed in 2003. I am still working on knowing what the users want and expect.

    4. Do you miss life at the bench?

    No!

    5. When is the last time you loaded a sample on the flow cytometer?

    What time is it now?



    Dept. of Immunology | School of Medicine | Pitt Home | FACS Facilities at Pitt | Contact Us

    Web site created by John Pome.