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  • 12/10/2013

  • ATTENTION: DO NOT SCHEDULE HERE USE SCHEDULEBOOK
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  • 6/25/2013

  • Free software for flow cytometry analysis.
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  • 3/20/2013

  • Mulitcolor Flow Cytometry Seminar March 26 1:00pm-3:00pm BST 10th floor conference room.
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  • 2/4/2013

  • Unexpected autofluorescence from certain bullet tubes.
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  • 1/8/2013

  • Mass Cytometer-CyTOF - special seminar sponsored by Starzl Inst.
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    News Archive...
           LSR II Reservations    |    Fortessa    |    Protocols     |    Sample Prep    |    Instrument Fees

    Tip #381. Backgating for confident FACS analysis.
    Not sure if your "positive" staining is real or is due to contamination by dead cells or autofluorescent cells?

    For an excellent example of how to use stringent gating, and intelligent backgating, to eliminate false positive staining see Vijaynand et al., NEJM (2007) 356:1410. See especially supplemental Figure E3 which shows you step by step how dead cells, doublets, and macrophages contribute to false PE/FITC staining in a tetramer analysis of airway NKT cells.


    Michelle Heid
    PhD Student
    Department of Immunology

    On trainee optimism in this NIH funding climate...

    1. What inspired you to pursue a PhD?

    I have enjoyed learning about science and conducting science projects since I was a child. This interest in science has grown throughout my academic career. Pursuing a PhD was the next step in moving forward toward my goal of a future career in science.

    2. How is your thesis work progressing?

    My thesis work is progressing well. I am excited about the way my project is coming together.

    3. It's a difficult time for trainees. NIH funding is tight and labs are shrinking. Yet many our recent graduates have found quality postdoctoral positions - including an amazing number that have ended up at the NIH. Is there a sense of optimism or pessimism among our graduate students?

    I feel like there is still a sense of optimism among the graduate students. While we acknowledge the current funding difficulties, we remain hopeful and are greatly encouraged by the success of recent graduates.

    4. Congratulations on your 2011 travel award to give an oral presentation at the annual American Association of Immunologists (AAI) meeting! How did it go?

    I think my talk flowed well and I am glad that I had the opportunity to present at a national meeting. I greatly enjoyed attending the meeting and seeing San Francisco for the first time.

    5. It was a thrill that, in total, eleven of our PhD candidates were invited to give first-authored presentations at AAI. From the graduate faculty perspective, we actively try to position our trainees for success. Do you have any suggestions for how we can improve the Program to maximize your opportunities?

    I think the program does a very good job at giving all the trainees opportunities. We have numerous chances to attend meetings and present our work. I think this is one of the programís greatest strengths.

    6. What is your favorite spot to relax in Pittsburgh?

    I donít have one favorite spot in Pittsburgh, but generally I like to relax in parks and wooded areas. There are also many areas close to Pittsburgh where I like to go hiking.


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