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  • 12/10/2013

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  • 6/25/2013

  • Free software for flow cytometry analysis.
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  • 3/20/2013

  • Mulitcolor Flow Cytometry Seminar March 26 1:00pm-3:00pm BST 10th floor conference room.
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  • 2/4/2013

  • Unexpected autofluorescence from certain bullet tubes.
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  • 1/8/2013

  • Mass Cytometer-CyTOF - special seminar sponsored by Starzl Inst.
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    News Archive...
           LSR II Reservations    |    Fortessa    |    Protocols     |    Sample Prep    |    Instrument Fees

    Tip #751. Autofluoresence and compensation.
    Autofluorescence is intrinsic cellular fluoresence that can contribute to background signals, and interfere with sensitive detection of low levels of antibody staining. For optimal resolution of stained populations, try to choose compensation controls that have the same level of autofluorescence. Compensation beads will not be the best choice because of their low intrinsic fluorescent properties.

    Lisa Borghesi
    Associate Professor & Director, Flow Cytometry Facility
    Department of Immunology

    On User frustration with limited instrument availability...

    1. It is very hard to reserve time on the LSRII flow cytometer. How is the Flow Facility addressing this congestion that impacts the quality and efficiency of my science?

    We are aware that our current instrumentation is limiting, and doesn't meet the needs of the growing FACS community. Moreover, the increasing demand is for complex multicolor flow cytometry, not just one or two color visualization. We have written equipment grant to the NIH to purchase a second flow cytometer. The proposed instrument is an LSRFortessa which is the latest version of our current LSRII just with a tidier footprint.

    2. When will you find out if the equipment grant funded?

    Our priority score is a 28. The missing information is the NIH payline, which we don't yet know. Following the recent 7% cut in discretionary spending associated with the debt-ceiling deal, NCRR (National Center for Research Resources) paylines are totally unclear. NIH Council meets in September, so we should know more then.

    3. What are my options if the instrument grant is not funded?

    Keep in mind the other flow facilities we have on campus. The Starzl Transplantation Institute Flow Facility, with whom we have a close relationship, has two instruments of similar configuration to our LSRII. In fact the Starzl group participated in our current NIH instrumentation grant, since procuring the instrument would help Users of both facilities.

    4. How do you and Dewayne coordinate your roles in the facility?

    I've never seen someone as patient and unflappable as Dewayne. I think it's because he has three kids! We make a good team. Whereas I get a bit undone when equipment goes down, Dewayne is unperturbed. Proof - number of times I have called Dewayne when he has been on vacation: 6. Number of times he has called me when I'm away: 0.

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